Overexpression of cationic amino acid transporter-1 increases nitric oxide production in hypoxic human pulmonary microvascular endothelial cells
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چکیده
منابع مشابه
Characterization of cationic amino acid transporters and expression of endothelial nitric oxide synthase in human placental microvascular endothelial cells.
We investigated the expression and activity of arginine transporters and endothelial nitric oxide synthase (eNOS) in human placental microvascular endothelial cells (HPMEC). Using RT-PCR amplification products for eNOS, CAT1, CAT2A, CAT2B, CAT4, 4F2hc (CD98), rBAT and the light chains y+LAT1, y+LAT2, and b0+T1 were detected in HPMEC, but not B0+. Immunohistochemistry and Western blotting confir...
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Inducible nitric oxide (NO) synthase (iNOS) is a stress response protein upregulated in inflammatory conditions, and NO may suppress cellular proliferation. We hypothesized that preventing L-arginine (L-arg) uptake in endothelial cells would prevent lipopolysaccharide/tumor necrosis factor-α (LPS/TNF)-induced, NO-mediated suppression of cellular proliferation. Bovine pulmonary arterial endothel...
متن کاملTransport of extracellular l-arginine via cationic amino acid transporter is required during in vivo endothelial nitric oxide production.
In cultured endothelial cells, 70-95% of extracellular l-arginine uptake has been attributed to the cationic amino acid transporter-1 protein (CAT-1). We tested the hypothesis that extracellular l-arginine entry into endothelial cells via CAT-1 plays a crucial role in endothelial nitric oxide (NO) production during in vivo conditions. Using l-lysine, the preferred amino acid transported by CAT-...
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It is now accepted that allicin, the main biologically active compound in garlic, exhibits antioxidant activity. The present study was designed to test the hypothesis that the antioxidant activity of garlic can be partially attributed to the inhibition of nitric oxide (NO) production by cytokine-induced NO synthase (iNOS). Cardiac myocytes cultured from neonatal Wistar rats were stimulated by l...
متن کاملThe Effect of Some Herbal Extracts on Nitric Oxide Production in Endothelial Cells 3T3 Cell Line
Introduction: Some studies have demonstrated the potential of herbal drugs for the treatment of various diseases associated with impaired vascular nitric oxide (NO). For diagnosis of the mechanism of these herbal plants, it seems necessary to evaluate the herbal extracts on the Endothelium cell. The aim of the study was to investigate the effect of extract of Trigonella foenum-graecum (T.foenum...
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ژورنال
عنوان ژورنال: Clinical and Experimental Pharmacology and Physiology
سال: 2011
ISSN: 0305-1870
DOI: 10.1111/j.1440-1681.2011.05609.x